核固红染色液（0.2%）

核固红染色液（0.2%）
货号：G1321
规格：100ml
英文名称 : NUCLEAR FAST RED
CAS : 6409-77-4

    核固红染色液是组织切片染色中常用的复染液，染色后细胞核呈红色。染色液可重复使用多次，但染色效果会逐渐降低。

核固红染色液使用方法：

1、 切片脱蜡水，可进行其他染色，染色后蒸馏水洗2-5min。

2、 将组织切片浸入核固红染色液，也可以直接滴加到样本上染色5-10分钟(浅染细胞核)，染色时间可根据染色深度做相应调整。

3 自来水中冲洗去除多余的染色液。

4、 常规脱水透明，中性树胶封固。

注意事项：

1、 切片脱蜡应尽量干净。

2、*次使用本试剂时建议先取1-2个样品做预实验。

3、染色过程推荐浅染，通常能够分辨细胞核即可，颜色过深有可能影响细胞颜色。

4、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Nuclear Fast Red
CAS Number: 6409-77-4
Storage Temperature: Room Temperature
Product Description :
Appearance: Dark Red to Very Dark Brown Powder
Molecular Formula: C14H8NNaO 7S
Molecular Weight: 357.3
Synonyms: Kernechtrot, Calcium Red
This dye has been used as a counterstain in saturated aqueous potassium alum and in a counterstain
solution of 0.1% (w/v) with 5% aluminum sulfate for a Prussian Blue Stain for ferric iron. It has been used
as a reagent for calcium in tissue where it forms a scarlet lake. Lakes are also formed with lead, ferric
ion, copper, tin, and strontium, but not barium. The suitability of nuclear fast red as a nuclear stain in
autoradiographs of knee joints of mice and rats has been reported.
Storage/Stability :
When stored at room temperature, this product is given a shelf-life of four years from the date of
manufacture.
Preparation Instructions:
This dye is reported to be soluble in water (6 mg/ml) and in ethanol (0.4 mg/ml), but it is likely that heat will
be required for the aqueous solution.
Procedure :
Preparation of a nuclear fast red (Kernechrot) solution for use as a counterstain:
1. Dissolve 25 g of aluminum sulfate in 500 ml of distilled water.
2. Add 0.5 g of nuclear fast red. Use heat to dissolve.
3. Cool, filter, and add a few grains of thymol as a pretive.
For use as a counterstain:
1. Rinse sections in distilled water.
2. Counterstain sections in nuclear-fast red solution for 5 minutes.
3. Wash in running tap water for 1 minute or more.
4. If using an aqueous mounting medium, the slides are ready to coverslip. If using a permanent
mounting medium, dehydrate in 2 changes of 95% alcohol, 2 changes of absolute ethanol, and 2 changes
of xylene. Slides can now be mounted using a synthetic, permanent mounting medium.

Note: Inadequate washing of the slides after nuclear fast red staining will result in cloudy slides. If the
slides have not been coverslipped in a permanent mounting medium, return to running water and wash
well. If slides have been coverslipped in a permanent mounting medium, soak in xylene to remove thecoverslip, soak in 3 changes of xylene to remove the mounting 

核固红染色液（0.2%）订购说明：
1、绝大部分产品备有现货，一般情况下都能订货当日发货。 
2、部分非常用产品，需提前1－2日预订，海外期货则需要提前3-6周预订。 
3、部分产品价格会因货期、批次等因素发生变化，若有变动以订货当日新价格为准。 
4、每日订单截止时间为16点整，部分城市可到17点整，因超过截止时间造成当日不能发货的将于次日安排发货。
5、请办理完货款后，将收据、底单等连同收货人的地址、姓名、等以传真、、短信、等形式通知我们。

运输说明：
1、极低温产品：极低温产品运输过程中加装干冰运输。用干冰把产品包裹起来，再用泡沫盒密封，胶带层层粘住泡沫盒，放入索莱宝的箱子，然后交付给合作快递，安全快速高效放心的送客户的手中，保证产品的性质稳定如一，为您的实验保驾护航。
2、低温产品：低温产品运输过程中加装索莱宝冰袋运输。事先用冰袋把产品包裹起来，再使用泡沫盒密封，用胶带严实密封泡沫盒，再放入索莱宝的箱子（保温效果少可以持续一周），然后交付给合作快递，安全快速高效放心的送客户的手中，保证产品的性质稳定如一，为您的实验保驾护航。
3、常温产品：常温产品运输过程中无需加冰或者特殊包装。产品由公司库房人员快速配货，准确快速高效的快递保证产品快速送达您的手中。