欢迎光临北京索莱宝科技有限公司网站!
销售咨询热线:
17801761073
您的位置: 网站首页 > 产品中心 > 分子生物学 > DNA电泳 > SY1040DNA电泳试剂 SYBR Green II(10000×)
  • DNA电泳试剂 SYBR Green II(10000×)
产品名称:

DNA电泳试剂 SYBR Green II(10000×)

产品品牌: SOLARBIO/索莱宝 价格:
厂商性质: 生产厂家 产品时间: 2024-03-23
DNA电泳试剂 SYBR Green II(10000×)
SYBR ® Green II RNA gel stain
Cat# :SY1020
Introduction SYBR

产品概述

品牌SOLARBIO/索莱宝

DNA电泳试剂 SYBR Green II(10000×)

SYBR ®  Green II RNA gel stain
Cat# :SY1020
Introduction
SYBR
®  Green II RNA gel stain is one of the most sensitive dyes known for detecting RNA in electrophoreticgels. As little as 100 pg RNA or single-stranded DNA per band can be detected in a SYBR Green IIBstained agarose or polyacrylamide gel using 254 nm epi-illumination Polaroid
®  667, black-and-white print film and a SYBR Green gel stain photographic filter.
Even with 300 nm transillumination, as little as 500 pg RNA per band can be detected. SYBR Green II RNA gel stain is significantly more sensitive than ethidium bromide,the most commonly used stain for detecting nucleic acids in gels (Figure 1). With 300 nmtransilluminationand photography through an orange-red gelatin filter, ethidium bromide’s sensitivity limit in a standardagarose minigel is about 1.5 ng single-stranded nucleic acid per band. Note that our detection limits arebased on results obtained with a FotoDyne
®  Foto/UV ®  450 ultraviolet transilluminator in combination with
Polaroid 667 film. Video cameras and CCD cameras in general have a different spectral response thanblack-and-white print film and thus may not exhibit the same sensitivity.
A BFigure 1. Dilution series of Escherichia coli ribosomal 16S and 23S RNA electrophoresed in 1% agarose gels. The gelscontain an identical twofold dilution series of RNA. The gel shown in panel A was stained for 20 minutes with SYBR Green II
RNA gel stain (using a 1:10,000 dilution of the stock reagent) and not destained. The gel shown in panel B was stained for 20minutes with 5 µg/mL ethidium bromide, then destained for an additional 20 minutes. The SYBR Green II dye-stained gel was excited using 254 nm epi-illumination and the ethidium bromide–stained gel using 300 nm transillumination (Fotodyne
®  FotoUV 450 ultraviolet transilluminator). Although the SYBR Green II dye-stained gel can be excited at 300 nm, epi-illumination at 254 nm resulted in the best sensitivity in our hands. Both gels were photographed with Polaroid 667 black-and-white print film,using a SYBR Green gel stain photographic filter (SYBR Green II dye-stained gel) or an ethidium bromide gel stainphotographic filter (ethidium bromide– stained gel).
SYBR Green II RNA gel stain can be used to:
 Analyze small aliquots from RNA preparations before Northern blotting, start-site mapping or cDNApreparation
 Visualize the migration behavior of 5S rRNA species after high-resolution denaturing gradienectrophoresis (DGGE)
Stain DNA in single-strand conformation polymorphism (SSCP)
Stain DNA before amplification by PCR
Materials Contents SYBR Green II stain is provided as a 10,000X concentrate in DMSO in the following sizes:
 50 µL (SY1040-50)
 500 µL (SY1040-500)
Storage
Upon receipt, store the dye frozen at -20°C, protected from light in a desiccator. When stored properly,the SYBR Green II stain stock solution in DMSO is stable for six months to one year.


注:SYBR Green II主要用于RNA和单链DNA电泳。是EB和替代品,无致癌性,超敏感性。用作常规电泳时,须将商品化的核酸MARKER作一定倍数的稀释(1/5-1/10),这样才能得到比较理想的结果。

 

DNA电泳试剂 SYBR Green II(10000×)订购说明:
1、绝大部分产品备有现货,一般情况下都能订货当日发货。 
2、部分非常用产品,需提前1-2日预订,海外期货则需要提前3-6周预订。 
3、部分产品价格会因货期、批次等因素发生变化,若有变动以订货当日新价格为准。 
4、每日订单截止时间为16点整,部分城市可到17点整,因超过截止时间造成当日不能发货的将于次日安排发货。
5、请办理完货款后,将收据、底单等连同收货人的地址、姓名、等以传真、、短信、等形式通知我们。


DNA电泳试剂 SYBR Green II(10000×)运输说明:
1、极低温产品:极低温产品运输过程中加装干冰运输。用干冰把产品包裹起来,再用泡沫盒密封,胶带层层粘住泡沫盒,放入索莱宝的箱子,然后交付给合作快递,安全快速高效放心的送客户的手中,保证产品的性质稳定如一,为您的实验保驾护航。
2、低温产品:低温产品运输过程中加装索莱宝冰袋运输。事先用冰袋把产品包裹起来,再使用泡沫盒密封,用胶带严实密封泡沫盒,再放入索莱宝的箱子(保温效果少可以持续一周),然后交付给合作快递,安全快速高效放心的送客户的手中,保证产品的性质稳定如一,为您的实验保驾护航。
3、常温产品:常温产品运输过程中无需加冰或者特殊包装。产品由公司库房人员快速配货,准确快速高效的快递保证产品快速送达您的手中。

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7