品牌 | SOLARBIO/索莱宝 | 货号 | K000137M |
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规格 | 50ul/100ul | 供货周期 | 现货 |
主要用途 | WB IP | 应用领域 | 医疗卫生,环保,化工,生物产业,农业 |
英文名称 | Anti-EGF Receptor Monoclonal Antibody |
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宿主 | Mouse |
别名 | ERBB;ERBB1;HER1;mENA;NISBD2;PIG61 |
应用 | WB IP |
稀释比例 | WB 1:2000. |
交叉反应 | Human |
蛋白分子量 | 175kDa |
Gene ID | 1956 |
保存 | Store at -20°C. Avoid freeze / thaw cycles. |
储存液 | Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
纯化方法 | Affinity purification |
亚型 | IgG1 |
免疫原 | Purified recombinant human EGFR protein fragments expressed in E.coli. |
性状 | 液体 |
Public Immunogen Range | Purified recombinant human EGFR protein fragments expressed in E.coli. |
Subcellular Locations | Cell membrane Endoplasmic reticulum membrane Endosome Endosome membrane Golgi apparatus membrane Nucleus membrane Nucleus Secreted Single-pass type I membrane protein |
Swiss Prot | P00533 |
克隆号 | 1A11-G9-1E9 |
克隆类型 | Monoclonal Antibody |
背景资料 | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010] |
单克隆抗体
抗原通常是由多个抗原决定簇组成的,由一种抗原决定簇刺激机体,由一个B淋巴细胞接受该抗原所产生的抗体称之为单克隆抗体(Monclone antibody).单克隆抗体自问世以来,由于其的特征已迅速应用于医学很多领域。例如作为检验医学诊断的试剂。因为单克隆抗体有特异性强、纯度高、均一性好的优点,因此可以广泛应用于酶联免疫吸附试验、放射免疫分析、免疫组化和流式细胞仪等技术;单克隆抗体还可用于蛋白质的提纯。单克隆抗体是亲和层析中重要的配体。将单克隆抗体吸附在一个惰性的固相基质上,并制备成层析柱。当样品流经层析柱时,待分离的抗原可与固相的单克隆抗体发生特异性结合,其余成分不能与之结合。将层析柱充分洗脱后,改变洗脱液的离子强度或pH,欲分离的抗原与抗体解离,收集洗脱液便可得到欲纯化的抗原;除此之外,单克隆抗体还有望于参与肿瘤的导向治疗和放射免疫显像技术,将针对某一肿瘤抗原的单克隆抗体与化疗药物或放疗物质连接,利用单克隆抗体的导向作用,将药物或放疗物质携带靶器官,直接杀伤靶细胞,称为肿瘤导向治疗。
单克隆抗体的优点