DMEM高糖，细胞培养

DMEM高糖，细胞培养

DMEM是现今细胞培养中比较常见的培养基之一，dmem培养基是建立在MEM培养基的基础上研究出来的，它与MEM培养基相比较，DMEM培养基中加大了MEM培养基中各种成分的用量，下文主要介绍一下自己配制DMEM的方法。

1 制备新鲜三蒸水或Millipore超纯水。

2 称取所需量的干粉培养基，加入约终体积一半的三蒸水中;若配制一个包装的培养液，在将整个包装的干粉倒入三蒸水后，需用水洗包装袋内面2次，倒入培养液中，以保证所有干粉都溶解成培养液。磁力搅拌或人工搅拌使之*溶解。

3 根据包装袋上的要求补加所需量的碳酸氢钠;根据实验需要，添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物质。

4 加水定容到终体积。

5 必要时用1 mol/L 盐酸和1 mol/L 氢氧化钠调节pH。

6 用无菌0.22um滤膜过滤除菌，分装于无菌血清瓶中，4℃冰箱保存。

配制好的培养液用前加入100U/mL青霉素和100U/mL链霉素，并根据需要加入血清(5%-20%)。 DMEM培养基应用广泛。基于DMEM培养基中氨基酸、微生物含量丰富，并具有糖酵解中的丙酮酸和微量Fe离子，因此在细胞培养、疫苗生产等方面的应用为常见。

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DMEM高糖，细胞培养