胺盐Spermine

胺盐Spermine

胺盐Spermine
Product Number S3256
Store at 2-8 °C
Product Description
Molecular Formula: C 10 H 26 N 4
Molecular Weight: 202.34
CAS Number: 71-44-3
Melting Point:  55 - 60 °C 1
28 - 30 °C 2
Synonym: N,N'-bis(3-aminopropyl)-1,4-butanediamineSpermine is a naturally occuring polyamine that occursin all eukaryotes, but is rare in prokaryotes. It isessential for cell growth in both normal and neoplastictissue. 1 Spermine is formed through the addition of aaminopropyl group to spermidine by sperminesynthase. Spermine is strongly basic in character, and in aqueous solution at physiological pH, all of its amino groups will be positively charged. 3 A review of the role of spermine and other polyamines in affecting RNA structure and protein function has been published. 4 Spermine is commonly used in molecular biology and biochemistry research. The polycationic character of spermine in solution allows for its use in the precipitation of DNA of >100 base pairs in length from low salt aqueous buffers, and for the isolation of DNA from pulse field gels. 5,6,7 Spermine has also been utilized in chromosome isolation and in the aggregation of chromatin. 8,9 It may be used as a building block for the preparation of gene transfer agents. 10,11 The complexation of spermine with DNA
to form particles with diameter <100 nm has been studied. 12 Spermine has been used in the crystallization of DNA. 13,14
It has been reported that spermine interacts with the glycine binding site associated with the N-methyl- D-aspartate receptor complex. 15 Spermine has been
shown to inhibit the conversion of L-arginine to L-citrulline by neuronal nitric oxide synthase in cytosolic preparations of rat cerebellum and cultured cerebellar granule neurons. 16 In acute hippocampal slices, 1 mM spermine exerts a neuroprotective effect
by blocking the NMDA receptor and voltage-activated Ca 2+ channels. 17 The effect of spermine on MK-801 binding to recombinant N-methyl-D-aspartate receptors has been investigated. 18 Proteins and protein complexes have been crystallized using spermine. 19,20 Other applications of spermine include its use as a matrix in MALDI-MS for analysis of glycoconjugates and oligonucleotides. 21,22 Precautions and Disclaimer For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions
This product is soluble in water (50 mg/ml), yielding a clear, colorless to light yellow solution. Storage/Stability Store at 2-8 °C. Solutions of spermine free base are readily oxidized. Solutions are most stable if prepared in degassed water and stored in frozen aliquots, under argon or nitrogen gas. References
1. The Index, 13th ed., Entry# 8817.
2. -Aldrich QC history for spermine.
3. Methods in Molecular Biology, Volume 79:Polyamine Protocols, Morgan, D. M. L., ed.Humana Press (Totowa, NJ: 1998), pp. 3-30.
4. Igarashi, K., and Kashiwagi, K., Polyamines:mysterious modulators of cellular functions.Biochem. Biophys. Res. Commun., 271(3), 559-564 (2000).
5. Basic Methods in Molecular Biology, 2nd ed.,Davis, L. G., et al., Appleton & Lang (Norwalk, CT:1994), pp. 179-180.
6. Wallace, D. M., Precipitation of nucleic acids.Methods Enzymol., 152, 41-48 (1987).
7. Molecular Cloning: A Laboratory Manual, 3rd ed.,Sambrook, J. and Russell, D. W., CSHL Press(Cold Spring Harbor, NY: 2001), pp. .5.86-5.88.
8. Cram, L. S., et al., Polyamine buffer for bivariatehuman flow cytogenetic analysis and sorting.Methods Cell Biol., 33, 377-382 (1990).
9. Marquet, R., et al., The condensation of chromatinand histone H1-depleted chromatin by spermine.J. Biomol. Struct. Dyn., 4(2), 205-218 (1986).
10. Ronsin, G., et al., Novel spermine-based cationic gemini surfactants for gene delivery. Chem.Commun. (Camb.), 21, 2234-2235 (2001).
11. Azzam, T., et al., Polysaccharide-oligoamine based conjugates for gene delivery. J. Med. Chem., 45(9), 1817-1824 (2002). 12. Trubetskoy, V. S., et al., The role of a microscopic colloidally stabilized phase in solubilizing oligoamine-condensed DNA complexes. Biophys. J., 84(2), 1124-1130 (2003).

13. Shui, X., et al., The B-DNA dodecamer at high resolution reveals a spine of water on sodium. Biochemistry, 37(23), 8341-8355 (1998).
14. Saminathan, M., et al., Polyamine structural effects on the induction and stabilization of liquid crystalline DNA: potential applications to DNA packaging, gene therapy and polyamine therapeutics. Nucleic Acids Res., 30(17), 3722- 3731 (2002).
15. Sacaan, A. I., and Johnson, K. M., Spermine enhances binding to the glycine site associated with the N-methyl-D-aspartate receptor complex. Mol. Pharmacol., 36(6), 836-839 (1989).
16. Hu, J., et al., Polyamines Inhibit Nitric Oxide Synthase in Rat Cerebellum. Neurosci. Lett., 175(1-2), 41-45 (1994).
17. Ferchmin, P. A., et al., Spermine is neuroprotective against anoxia and N-methyl-D- aspartate in hippocampal slices. Brain Res., 859(2), 273-279 (2000).
18. Sharma, T. A., and Reynolds, I. J., Characterization of the effects of polyamines on
[ 125 I]MK-801 binding to recombinant N-methyl-D- aspartate receptors. J. Pharmacol. Exp. Ther., 289(2), 1041-1047 (1999).
19. Xie, X., et al., Coiled-coil packing in spermine- induced tropomyosin crystals. A comparative study of three forms. J. Mol. Biol., 236(4), 1212- 1226 (1994).
20. Qian, C., et al., A rational approach towards successful crystallization and crystal treatment of human cytomegalovirus protease and its inhibitor complex. Acta Crystallogr. D Biol. Crystallogr., 56 (Pt 2), 175-180 (2000).
21. Mechref, Y., and Novotny, M. V., Matrix-assisted laser desorption/ionization mass spectrometry of acidic glycoconjugates facilitated by the use of spermine as a co-matrix. J. Am. Soc. Mass Spectrom., 9(12), 1293-1302 (1998).
22. Asara, J. M., and Allison, J., Enhanced detection of oligonucleotides in UV MALDI MS using the tetraamine spermine as a matrix additive. Anal. Chem., 71(14), 2866-2870 (1999). GCY / RXR 1/06

产品订购说明：
1、绝大部分产品备有现货，一般情况下都能订货当日发货。 
2、部分非常用产品，需提前1－2日预订，海外期货则需要提前3-6周预订。 
3、部分产品价格会因货期、批次等因素发生变化，若有变动以订货当日新价格为准。 
4、每日订单截止时间为16点整，部分城市可到17点整，因超过截止时间造成当日不能发货的将于次日安排发货。
5、请办理完货款后，将收据、底单等连同收货人的地址、姓名、等以传真、、短信、等形式通知我们。
产品运输说明：
极低温产品：极低温产品运输过程中加装干冰运输。用干冰把产品包裹起来，再用泡沫盒密封，胶带层层粘住泡沫盒，放入索莱宝的箱子，然后交付给合作快递，安全快速高效放心的送客户的手中，保证产品的性质稳定如一，为您的实验保驾护航。
低温产品：低温产品运输过程中加装索莱宝冰袋运输。事先用冰袋把产品包裹起来，再使用泡沫盒密封，用胶带严实密封泡沫盒，再放入索莱宝的箱子（保温效果少可以持续一周），然后交付给合作快递，安全快速高效放心的送客户的手中，保证产品的性质稳定如一，为您的实验保驾护航。
常温产品：常温产品运输过程中无需加冰或者特殊包装。产品由公司库房人员快速配货，准确快速高效的快递保证产品快速送达您的手中。
公司简介：
北京索莱宝科技有限公司始创于2000年，是一家集产品研发、生产、销售、服务于一体的大型高科技生物企业。
公司总部坐落于北京中关村科技园区，拥有1200平米研发生产中心，2000平米仓储基地，并配有的物流系统。同时在全国30多个省市设有分公司及直销、分销机构。服务网络覆盖全国，是国内大的生命科学研发试剂供应商之一。并通过阿里巴巴将产品输入美、日、欧等发达国家，得到了市场的高度认可。
目前公司提供的产品近万种，超过5000种常备现货，涵盖分子生物学、细胞生物学、免疫学、生物医学等相关领域，并不断推陈出新扩充种类，为用户提供、专业的产品。公司拥有数十名由博、硕士组建的专业研发技术团队，并聘请了众多国内专家担任技术顾问。严格按照G M P和I SO 9000标准生产和管理，致力于为用户提供试剂，完善的解决方案，致力于打造*的民族品牌。“为科研服务，为生命尽责”。是每一个索莱宝人发自内心的服务理念。同时，我们建立了一套的企业-高校合作模式，共同利用彼此平台和优势，互通有无，并为高校学生提供各项实验技能培训，实现双赢。此时，我们也期待能与更多科研单位深入合作，共同为中国生命科学研究的发展而努力，互惠共赢。
Solarbio品牌的诞生，是中国民族企业在生命科学领域的一次大胆挑战，面对几乎被外国企业垄断的市场，面对严酷的竞争环境，索莱宝人依然坚定不移的走自主品牌战略，立足国内，放眼，为中国生命科学产业的发展默默的付出自己的努力。同时，我们也相信，不久的未来中国民族生物企业也一定能够屹立于世界！