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  • 细胞培养 RPMI Medium 1640
产品名称:

细胞培养 RPMI Medium 1640

产品品牌: 索莱宝 价格: 100
厂商性质: 生产厂家 产品时间: 2019-11-29
细胞培养 RPMI Medium 1640
RPMI 1640,含D-葡萄糖、碳酸氢钠、丙酮酸钠、HEPES 和 L-谷氨酰胺英文名称:RPMI Medium 1640CAS:的性质、化学式、分子式、比重、密度,同时也包括的沸点、熔点、MSDS、用途、作用、毒性、价格、生产厂家、

产品概述

品牌SOLARBIO/索莱宝货号11875
规格500ml供货周期现货
主要用途细胞培养试剂应用领域医疗卫生,化工,生物产业,石油

细胞培养 RPMI Medium 1640

平时研究用的细胞,有需要冷冻保存。冷冻的过程称为冻存,把冻上的细胞化开的过程叫细胞复苏。细胞复苏是一简单的试验操作,但操作中有许多需要注意的事项,在实验操作中注意细小问题,才能使复苏后的细胞状态保持良好。

细胞复苏实验准备:

主要器材:一次性滴管、打火机、酒精灯、大镊子、中镊子、记号笔、废液缸、封口膜、剪子。

主要试剂: PBS、培养液、消化酶(胰酶)、75%酒精、双抗。

PBS配制:NaCL :4g   KCL:0.1g   Na2HPO4 :1.745g   KH2PO4 :0.1g   

三蒸水: 500mL,溶解后高压灭菌。

培养基配制:5mL双抗、50mL血清,加入培养基中。

细胞复苏操作步骤:

1.佩戴眼镜和手套,从液氮罐中取出安瓿或冷冻管。

2.迅速放入38℃水浴中,并不时摇动,在1分钟内使其完全融化,然后在无菌下取出细胞。

3.在1000r/min速度下离心5~10分钟,弃去上层液,加入适量培养液后接种于培养瓶中,接种浓度1×109/L,置37℃温箱静置培养,次日更换一次培养液,继续培养,观察生长情况。若细胞密度较高,及时传代。或无需离心直接将细胞加入瓶中,并加入培养基贴壁培养12~24小时后,充去上清,换入新鲜培养基继续培养。

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细胞培养 RPMI Medium 1640

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