sciencell 内皮细胞培养基ECM

sciencell  内皮细胞培养基ECM

Endothelial Cell Medium(ECM)

Catalog Number: 1001

Product Description

Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human  microvascular endothelial  cells  in vitro.  It is a sterile, liquid medium  which  contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in  an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.

Components

ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of  endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of  penicillin/streptomycin solution (P/S, Cat. No. 0503). 

Product Use

ECM is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Store the basal medium at 4℃, the ECGS, the FBS and the P/S solution at -20℃. Protect from light. 

Shipping

Gel ice.

Prepare for use

Thaw  ECGS, FBS and P/S solution at 37℃.  Gently tilt the  ECGS tube several times during thawing  to help the contents dissolve.  Make sure the contents of the supplement are compley dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add ECGS, FBS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37℃. When stored in the dark at 4℃, the reconstituted medium is stable for one month.

Caution:

If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

sciencell  内皮细胞培养基ECM订购说明：

1、绝大部分产品备有现货，一般情况下都能订货当日发货。 
2、部分非常用产品，需提前1－2日预订，海外期货则需要提前3-6周预订。 
3、部分产品价格会因货期、批次等因素发生变化，若有变动以订货当日新价格为准。 
4、每日订单截止时间为16点整，部分城市可到17点整，因超过截止时间造成当日不能发货的将于次日安排发货。
5、请办理完货款后，将收据、底单等连同收货人的地址、姓名、等以传真、、短信、等形式通知我们。

sciencell  内皮细胞培养基ECM运输说明：

极低温产品：极低温产品运输过程中加装干冰运输。用干冰把产品包裹起来，再用泡沫盒密封，胶带层层粘住泡沫盒，放入索莱宝的箱子，然后交付给合作快递，安全快速高效放心的送客户的手中，保证产品的性质稳定如一，为您的实验保驾护航。
低温产品：低温产品运输过程中加装索莱宝冰袋运输。事先用冰袋把产品包裹起来，再使用泡沫盒密封，用胶带严实密封泡沫盒，再放入索莱宝的箱子（保温效果少可以持续一周），然后交付给合作快递，安全快速高效放心的送客户的手中，保证产品的性质稳定如一，为您的实验保驾护航。
常温产品：常温产品运输过程中无需加冰或者特殊包装。产品由公司库房人员快速配货，准确快速高效的快递保证产品快速送达您的手中。